Analysis of BIOLOG GN Substrate Utilization Patterns by Microbial Communities
نویسندگان
چکیده
منابع مشابه
Analysis of BIOLOG GN Substrate Utilization Patterns by Microbial Communities.
BIOLOG GN plates are increasingly used to characterize microbial communities by determining the ability of the communities to oxidize various carbon sources. Studies were done to determine whether the BIOLOG GN plate assay accurately reflects the catabolic potential of the inoculum used. To gain insight into which populations of microbial communities contribute to the BIOLOG patterns, denaturin...
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Characterization of community-level substrate utilization via incubation of environmental samples in BIOLOG microplates has been increasingly applied in many areas of microbial ecology. The easily-produced multivariate profile allows for intensive spatial and temporal analysis of microbial communities, and it is a sensitive and reproducible tool for characterizing microbial communities. This wo...
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Microbial growth on and utilization of environmental contaminants as substrates have been studied by many researchers. Most times, substrate utilization results in removal of chemical contaminant, increase in microbial biomass and subsequent biodegradation of the contaminant. These are all aimed at detoxification of the environmental pollutants. Several microbial growth and biodegradation kinet...
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Carbon source utilization tests based on the reducdefining a coloration threshold for positive controls tion of a tetrazolium dye were developed for the or a certain average absorption value improves identification of bacteria [1]. Since Garland and reproducibility, but which threshold gives the best Mills [2] first used such Biolog (Hayward, Ca., resolution? It was also proposed to determine ...
متن کاملThe Biolog Plates Technique as a Tool in Ecological Studies of Microbial Communities
The Biolog technique was introduced into ecological studies to estimate metabolic potential of microbial communities. while utilizing carbon substrates (95 or 31x3, depending on the plate type), microbes reduce a colourless dye to violet formazan. The colour is measured spectrophotometrically. This is a rapid and quite convenient method but it has many drawbacks – for example, only microbes tha...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1998
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.64.4.1220-1225.1998